Table of Contents

Pre-training and post-training evaluation

The participents will be evaluated with OSPE for skills and with MCQ for knowledge

Skill evaluation

Objective

Pippette 1000 microliter reagent using two step pippetting and discard tip into correct waste container

Steps of the procedure

Serial numberDescriptionMaximum MarksMarking instructionsMarks obtained
1Take 1000 microliter pippette2Wrong pippette choosen=0
Correct pippette choosen after trial=1
Correct pippette choosen at first attampt=2
2Apply correct tip over the pippette2Could not choose tip=0
choosen wrong tip initially=1
choosen correct tip on first attampt=2
Tip was appearantly loose=0
3Press pushbutton up to first resistance1Pushed correctly=1
Pusher more or less=0
4Dip tip in to beaker with liquid to be pippetted1<0.5 cm depth=1.
>0.5 cm depth=0
5Release push button2No bubble at first attampt=2
No bubble after multiple attampts=1
Bubble was ignored=0
6Position pippette tip over plastic eppindorf cup2Not touching wall=1
touching wall=2
7Press pushbutton up to second resistance2Pushed up to 2nd resistance=2
Pushed upto first resistance, with almost complate delivery=1
8Release pushbutton after lifting pippette away from delivery container2Lifted and released=2
not lifted but no re-aspiration of liquid=1
Liquid reaspirated during release=0
9Discard tip into red container2Not discarded=0
discarded in wrong container=1
discarded in correct container
Total Maximum Marks16

Required Materials and preparation of station

See above points and list all material required and arrange them on station

Knowledge Evaluation

Choose single best answer from the options given

1. Which of the following is a micropipette?

a
b
c
d

2. A micropipette which can take 50 to 500 microliter volume in step of 10 is called

a50 microliter fixed volume micropipette
b500 microliter fixed volume micropipette
cstep pipette
dvariable volume pipette

3. Following is not required during pipetting serum with micropipette

amicropipette
btip
cgloves
dsyringe

3. Micropitte have following push-button stops

a1
b2
c3
d4

4. Removal of tips from micropipette require ideally

a1 hand
b2 hands
cnone of the above
dall of the above

5. Plastic tips should be discarded in

ared bag
bblue bag
cblack bag
dyellow bag