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Department of Biochemistry, NCHS and GMCS

Majura Gate, Surat

NABL Accreditationexamination_idnamesample_requirementinstruction_for_requesterunits/reference_range/methodclass_tag
yes505924 hours Urine ProteinPlain-Urinemg(Calculated),Urine,NABL
5183ACR (Albumin-creatinine ratio)(not in NABL scope)Plain-Urine(Calculated)(mg/gm) Normal<30, Microalbuminuria 30-300, Macroalbuminuria >300,Urine
yes9241ALTBBPlain-BloodU/L <45 (L-Alanine LDH UV Kinetic),Blood,NABL
5032Adenosine Deaminase(not in NABL scope)Plain-CSFU/mL <15 (Berthelot),CSF
5139Adenosine Deaminase(not in NABL scope)Plain-OtherU/mL, Method:Berthelot, No Ref.Interval provided
5033Adenosine Deaminase(not in NABL scope)Plain-Peritoneal_FluidU/mL N/A (Berthelot)
5034Adenosine Deaminase(not in NABL scope)Plain-Pleural_FluidU/mL N/A (Berthelot)
yes5006Alanine TransaminasePlain-BloodU/L <45 (L-Alanine LDH UV Kinetic),Blood,NABL
yes5011AlbuminPlain-Blood g/dL 3.5-5.2 (BCG),Blood,NABL
5181Albumin(not in NABL scope)Plain-Peritoneal_Fluid g/dL N/A (BCG)
yes5007Alkaline PhosphatasePlain-BloodU/L 42-128 (pNPP with AMP buffer),Blood,NABL
yes5008AmylasePlain-BloodU/L 28-100 (CNPG),Blood,NABL
5106Amylase(not in NABL scope)Plain-OtherU/L NA(CNPG)
5040Amylase(not in NABL scope)Plain-Peritoneal_FluidU/L NA(CNPG)
5044Amylase(not in NABL scope)Plain-Pleural_FluidU/L NA(CNPG)
yes5013Aspartate transaminasePlain-BloodU/L <35 ( UV Kinetic),Blood,NABL
5133B-hCG(not in NABL scope)Plain-BloodIU/L, <=25, (ECIA),Blood
yes5065Band at HbA positionEDTA-BloodAP Level Reference required,Blood,NABL,AP-REF
yes5068Band at HbA2 positionEDTA-BloodAP Level Reference required,Blood,NABL,AP-REF
yes5066Band at HbF positionEDTA-BloodAP Level Reference required,Blood,NABL,AP-REF
yes5067Band at HbS positionEDTA-BloodAP Level Reference required,Blood,NABL,AP-REF
yes5079Band at M positionPlain-Blood,Blood,NABL
yes5092CKPlain-BloodU/L 25-125 (phosphocreatine HK G6PD),Blood,NABL
yes5093CK-MBPlain-BloodU/L 0-24 (Immunoinhibition, HK, G6PD),Blood,NABL
yes5121CRPPlain-Bloodmg/L, Ref. Interval: <10
Method: Immunoturbidimetry
Note:
This is not hsCRP. Do not use this result for interpretation when result is less than 10 		,Blood,NABL
yes5014CalciumPlain-Bloodmg/dL 8.6-10.2 (Arsenazo III),Blood,NABL
yes5050CalciumPlain-Urine mg/dL N/A (Arsenazo III),Urine,NABL
yes5057Calcium:creatinine RatioPlain-Urine(Calculated)(mg/mg),Urine,NABL
5123Cardiac Troponin I (cTnI)(not in NABL scope)Plain-Bloodng/L Male: 8.8-23.7 Female: 4.4-21.4 (high sensitivity, ECIA),Blood
5109Cholesterol(not in NABL scope)Plain-Other mg/dL N/A(CHOD-POD)
5042Cholesterol(not in NABL scope)Plain-Peritoneal_Fluid mg/dL N/A(CHOD-POD)
yes5113CholinesterasePlain-Blood U/L <3000-13000 (Butyrylthiocholine),Blood,NABL
5151Cl-Heparinised-Arterial_Bloodmmol/L 101-110 (Potentiometry using ISE),Blood
5215Cl-Heparinised-Venous_Bloodmmol/L (Direct ISE),Blood
yes5101Corrected CalciumPlain-Blood(Calculated)(mg/dL)8.6 - 10.2,Blood,NABL
yes5001CreatininePlain-Bloodmg/dL
(Jaffe two point)
Male: 0.9-1.3
Female: 0.6-1.1

,Blood,NABL
yes5052CreatininePlain-Urine mg/dL N/A (Jaffe two point),Urine,NABL
5094Creatinine(not in NABL scope)Plain-Peritoneal_Fluid mg/dL N/A (Jaffe two point)
5122D-dimer(not in NABL scope)Citrate-Bloodmicrogram/L , <500 , (ECIA),Blood
yes5010Direct BillirubinPlain-Bloodmg/dL <0.4 (Diazo Reaction),Blood,NABL
yes5071Dithonite test with UreaEDTA-BloodAP Level Reference required,Blood,NABL,AP-REF
yes5070Dithonite test without UreaEDTA-BloodAP Level Reference required,Blood,NABL,AP-REF
5130FSH(not in NABL scope)Plain-BloodIU/L (ECIA)
normal female follicular phase => 1.98-11.6
normal female midcycle peak=>5.14-23.4
normal female lueal phase => 1.38-9.58
post menopausal female => 21.5- 131
Normal male=> 1.555-9.74		,Blood
5118Ferritin(not in NABL scope)Plain-Bloodmicrogram/L Male(Adult): 20-250, Female(Adult): 10-120, Newborn: 25-200, 1 month: 200-600, 2-5 month:50-200, 6 month-15 years:7-140 (ECLIA) ,Blood
5128Free T3(not in NABL scope)Plain-BloodAP Level Reference required pmol/L 4.26-8.10 (ECIA),Blood
5127Free T4(not in NABL scope)Plain-BloodAP Level Reference required pmol/L 10.0-28.2 (ECIA),Blood
yes5031GlucoseFluoride-BloodUnit: mg/dL
[mmol/L Glucose] = [mg/dL Glucose] / 18

Criteria for the diagnosis of diabetes (any one of below)

  1. FPG >=126 mg/dL (7.0 mmol/L). Fasting is defined as no caloric intake for at least 8 h.*
  2. 2-h PG >=200 mg/dL (11.1 mmol/L) during OGTT. The test should be performed as described by WHO, using a glucose load containing the equivalent of 75 g anhydrous glucose dissolved in water.*
  3. A1C >=6.5% (48 mmol/mol). The test should be performed in a laboratory using a method that is NGSP certified and standardized to the DCCT assay.*
  4. In a patient with classic symptoms of hyperglycemia or hyperglycemic crisis, a random plasma glucose >=200 mg/dL (11.1 mmol/L).

Note1: DCCT, Diabetes Control and Complications Trial; FPG, fasting plasma glucose; OGTT, oral glucose tolerance test; NGSP, National Glycohemoglobin Standardization Program; WHO, World Health Organization; 2-h PG, 2-h plasma glucose.

Note2: *In the absence of unequivocal hyperglycemia, diagnosis requires two abnormal test results from the same sample or in two separate test samples.

,Blood,NABL
5212GlucosePlain-Blood,Blood
yes5029GlucosePlain-CSFmg/dl 40-70 (GOD-POD),CSF,NABL
yes5108GlucosePlain-Othermg/dl NA(GOD-POD),NABL
yes5035GlucosePlain-Peritoneal_Fluidmg/dl NA(GOD-POD),NABL
yes5036GlucosePlain-Pleural_Fluidmg/dl NA(GOD-POD),NABL
5136Glucose(not in NABL scope)Plain-Urine mg/dl(GOD-POD endpoint),Urine
105046H+Heparinised-Arterial_Bloodnmol/L 35.48-44.67 (Calculated),Blood
105047H+Heparinised-Venous_Bloodnmol/L 37.15-50.12 (Calculated),Blood
5154HCO3 Heparinised-Arterial_Bloodmmol/L 22.0-28.0 (Calculated),Blood
5187HCO3 Heparinised-Venous_Bloodmmol/L 23.0-29.0 (Calculated),Blood
yes5016HDL CholesterolPlain-Blood mg/dL >40 (Dextransulphate-Mg2+precipitat),Blood,NABL
yes5174HbA1cEDTA-BloodAP Level reference Required
Method: HPLCUnits: % of total adult hemoglobin
ResultProbable Diagnosis
<5.7Normal
5.7-6.4Prediabetes Mellitus
>6.4Diabetes Mellitus
Note: Values above are for diagnosis of Diabetes Mellitus only
If you are already diagnosed/treated for Diabetes Mellitus, goal for most people is 7% or less.
However, this goal will depend on many things such as your age and any other medical conditions.
Work with your doctor to set your own individual A1C goal.
,Blood,NABL,AP-REF
5216HbA1c(IFCC)EDTA-BloodAP Level reference Requiredmmol/mol(Calculated)
<38.78=normal
38.78-46.43=prediabetes
>46.43=diabetes		,Blood,NABL
5146HematocritHeparinised-Arterial_BloodUnit: %, Method: Impedance Conductimetry
Male: 41-50
Female: 36-48< 		,Blood
105050HematocritHeparinised-Venous_BloodUnit: %, Method: Impedance Conductimetry
Male: 41-50
Female: 36-48< 		,Blood
5160HemoglobinHeparinised-Arterial_BloodUnit: gm/dL, Method: Calculation
Male: 13.2 - 16.6
Female: 11.6 - 15
,Blood
105051HemoglobinHeparinised-Venous_BloodUnit: gm/dL, Method: Calculation
Male: 13.2 - 16.6
Female: 11.6 - 15
,Blood
yes5024Indirect BillirubinPlain-Blood(Calculated)(mg/dL)<1.3,Blood,NABL
5150Ionized Calcium(Ca2+)Heparinised-Arterial_Bloodmmol/L 1.15-1.30 (Potentiometry using ISE),Blood
5192Ionized Calcium(Ca2+)Heparinised-Venous_Bloodmmol/L 1.14-1.29 (Direct ISE),Blood
yes5021IronPlain-Bloodmicrogm/dL 41-141 (Ferrozine),Blood,NABL
5149K+Heparinised-Arterial_Bloodmmol/L 3.5-5.1 (Potentiometry using ISE),Blood
5189K+Heparinised-Venous_Bloodmmol/L 3.5-5.1 (Direct ISE),Blood
yes5102Ketone bodyPlain-Blood (Nitropruside),Blood,NABL
yes5054Ketone bodyPlain-Urine (Nitropruside),Urine,NABL
yes1099LDL CholesterolPlain-Bloodmg/dL <=130 (Calculated) Maureen Sampson Equation,Blood,NABL
yes5017LDL CholesterolPlain-Blood mg/dL <130 (Calculated) Friedewald's formula,Blood,NABL
5131LH(not in NABL scope)Plain-BloodIU/L
Normal female follicular phase ≥ 2.58-12.1
Normal female mid-cycle peak ≥ 27.3-96.9
Normal female luteal phase ≥ 0.833-15.5
Post menopausal female ≥ 13.1- 86.5 (ECIA)		,Blood
yes5025Lactate DehydrogenasePlain-BloodU/L <360 (L to P UV Kinetic),Blood,NABL
5047Lactate Dehydrogenase(not in NABL scope)Plain-CSFU/L NA(L to P UV Kinetic),CSF
5105Lactate Dehydrogenase(not in NABL scope)Plain-OtherU/L NA(L to P UV Kinetic)
5041Lactate Dehydrogenase(not in NABL scope)Plain-Peritoneal_FluidU/L NA(L to P UV Kinetic)
5046Lactate Dehydrogenase(not in NABL scope)Plain-Pleural_FluidU/L NA(L to P UV Kinetic)
yes5026Lipase(not in NABL scope)Plain-Blood U/L 0-5 (Turbidimetry ),Blood,NABL
5107Lipase(not in NABL scope)Plain-OtherU/L NA (Turbidimetry)
5039Lipase(not in NABL scope)Plain-Peritoneal_FluidU/L NA (Turbidimetry)
5045Lipase(not in NABL scope)Plain-Pleural_FluidU/L NA (Turbidimetry)
9242MPRBPlain-Bloodmg/dL NA(Pyrogallol Red)
yes5132MagnesiumPlain-Blood mg/dL 1.7-2.7 (Xylidyl blue),Blood,NABL
5111Micro Protein(not in NABL scope)Plain-Othermg/dL NA(Pyrogallol Red)
5125NT-ProBNP(not in NABL scope)Plain-BloodAP Level reference requiredpg/ml (ECIA)
Result Age Interpretation
<300 Any age Heart Failure Unlikely
>450 20-50 Yrs Heart Failure Likely
>900 50-75 Yrs Heart Failure Likely
>1800 >75 Yrs Heart Failure Likely
,Blood,AP-REF
5148Na+Heparinised-Arterial_Bloodmmol/L 136-145 (Potentiometry using ISE),Blood
5188Na+Heparinised-Venous_Bloodmmol/L 136-145 (Direct ISE),Blood
yes5069Other BandsEDTA-BloodAP Level Reference required,Blood,NABL,AP-REF
yes5100PhosphorusPlain-Blood mg/dL 2.5-4.5 (Ammonium Molybdate),Blood,NABL
yes5051PhosphorusPlain-Urine mg/dL N/A (Ammonium Molybdate),Urine,NABL
yes5020PotassiumPlain-Blood mmol/L 3.5-5.1 (Direct ISE),Blood,NABL
5219Potassium(not in NABL scope)Plain-Other mmol/L N/A(Direct ISE),Other
5173Potassium(not in NABL scope)Plain-Urine mmol/L N/A(Direct ISE),Urine
5049Potassium(not in NABL scope)Plain-Urine mmol/L N/A(Direct ISE),Urine
5129Prolactin (Not in NABL Scope)Plain-Bloodng/mL
Normal Nonpregnant Female: 3.0-18.6
Male: 3.7-17.9 (ECIA)		,Blood
yes5062Protein ElectrophoresisPlain-BloodAP Level reference required,Blood,NABL,AP-REF
5086Protein:creatinine Ratio (Not in NABL Scope)Plain-Urine(Calculated)(mg/mg),Urine
yes5061Serum OsmolalityPlain-Blood(Calculated),Blood,NABL
yes5019SodiumPlain-Blood mmol/L 136-145 (Direct ISE),Other,NABL
yes5048SodiumPlain-Urine mmol/L N/A(Direct ISE),Urine,NABL
5083Sodium(not in NABL scope)Plain-CSF mmol/L N/A(Direct ISE),CSF
5218Sodium(not in NABL scope)Plain-Other mmol/L N/A(Direct ISE),Other
yes5022TIBCPlain-Bloodmicrogm/dL 251-406(calculated),Blood,NABL
yes5117TSHPlain-BloodmIU/L (CLIA), 0.465-4.68 ,Blood,NABL
5141Total 25-OH Vit-D(not in NABL scope)Plain-BloodAP Level reference requirednmol/L (ECIA)
condition result
Deficient<50
Insufficient50-75
Sufficient75-250
potential toxicity >250
Multiply nmol/L result with 0.4 to convert it into ng/ml
,Blood,AP-REF
yes5009Total BillirubinPlain-Bloodmg/dL <1.3 (Diazo Reaction),Blood,NABL
yes5015Total CholesterolPlain-Blood mg/dL <200 (CHOD-POD),Blood,NABL
yes5012Total ProteinPlain-Blood g/dL 6.4-8.3 (Biuret),Blood,NABL
yes5030Total ProteinPlain-CSFmg/dL 15-40 (Pyrogallol red),CSF,NABL
yes5080Total ProteinPlain-CSFg/dL 0.015-0.040 (Biuret),CSF,NABL
yes5038Total ProteinPlain-Peritoneal_Fluidg/dL NA(Biuret),NABL
yes5082Total ProteinPlain-Peritoneal_Fluidmg/dL NA(Pyrogallol Red),NABL
yes5037Total ProteinPlain-Pleural_Fluidg/dL NA(Biuret),NABL
yes5081Total ProteinPlain-Pleural_Fluidmg/dL NA(Pyrogallol Red),NABL
yes5053Total ProteinPlain-Urineg/dL N/A (Biuret),Urine,NABL
yes5085Total ProteinPlain-Urinemg/dL NA(Pyrogallol Red),Urine,NABL
5112Total Protein(not in NABL scope)Plain-Other g/dL N/A (Biuret)
yes5018TriglyceridePlain-Bloodmg/dL <150 (LipaseGPOPOD),Blood,NABL
5110Triglyceride(not in NABL scope)Plain-Other mg/dL N/A(Lipase GPO - POD)
5043Triglyceride(not in NABL scope)Plain-Peritoneal_Fluid mg/dL N/A(Lipase GPO - POD)
yes5023UIBCPlain-Bloodμgm/dL 120-470((Ferrozine Alkaline PH),Blood,NABL
yes5002UreaPlain-Bloodmg/dL 13-43 (Urease GLDH),Blood,NABL
5135Urea(not in NABL scope)Plain-Urine mg/dl(urease GLDH),Urine
5208Urea(not in NABL scope)Plain-Urine mg/dl(urease GLDH),Urine
yes5027Uric AcidPlain-Bloodmg/dL 2.6-7.2 (Uricase POD),Blood,NABL
yes5055Uric AcidPlain-Urinemg/dL N/A (Uricase POD),Urine,NABL
yes5028VLDL CholesterolPlain-Blood mg/dL <30 (Calculated),Blood,NABL
5142Vitamin B12(not in NABL scope)Plain-BloodAP Level reference requiredpmol/L 176-687 \n(ECIA),Blood,AP-REF
yes5060eGFRPlain-BloodmL/min/1.73 m²(Calculated)(MDRD),Blood,NABL
5144pCO2Heparinised-Arterial_BloodmmHg 32-48 (Potentiometry using ISE),Blood
5185pCO2Heparinised-Venous_BloodmmHg 38-58 (Potentiometry using ISE)),Blood
5143pHHeparinised-Arterial_Blood7.35-7.45 (Potentiometry using ISE),Blood
5184pHHeparinised-Venous_Blood7.30-7.43 (Potentiometry using ISE)),Blood
5145pO2Heparinised-Arterial_BloodmmHg 83-108 (Amperometry),Blood
5186pO2Heparinised-Venous_BloodmmHg 19-65 ((Amperometry),Blood