Pre-training and post-training evaluation
The participents will be evaluated with OSPE for skills and with MCQ for knowledge
Skill evaluation
Objective
Pippette 1000 microliter reagent using two step pippetting and discard tip into correct waste container
Steps of the procedure
| Serial number | Description | Maximum Marks | Marking instructions | Marks obtained |
|---|---|---|---|---|
| 1 | Take 1000 microliter pippette | 2 | Wrong pippette choosen=0 Correct pippette choosen after trial=1 Correct pippette choosen at first attampt=2 | |
| 2 | Apply correct tip over the pippette | 2 | Could not choose tip=0 choosen wrong tip initially=1 choosen correct tip on first attampt=2 Tip was appearantly loose=0 | |
| 3 | Press pushbutton up to first resistance | 1 | Pushed correctly=1 Pusher more or less=0 | |
| 4 | Dip tip in to beaker with liquid to be pippetted | 1 | <0.5 cm depth=1. >0.5 cm depth=0 | |
| 5 | Release push button | 2 | No bubble at first attampt=2 No bubble after multiple attampts=1 Bubble was ignored=0 | |
| 6 | Position pippette tip over plastic eppindorf cup | 2 | Not touching wall=1 touching wall=2 | |
| 7 | Press pushbutton up to second resistance | 2 | Pushed up to 2nd resistance=2 Pushed upto first resistance, with almost complate delivery=1 | |
| 8 | Release pushbutton after lifting pippette away from delivery container | 2 | Lifted and released=2 not lifted but no re-aspiration of liquid=1 Liquid reaspirated during release=0 | |
| 9 | Discard tip into red container | 2 | Not discarded=0 discarded in wrong container=1 discarded in correct container | |
| Total Maximum Marks | 16 |
Required Materials and preparation of station
See above points and list all material required and arrange them on station
Knowledge Evaluation
Choose single best answer from the options given
1. Which of the following is a micropipette?
| a |  |
| b |  |
| c |  |
| d |  |
2. A micropipette which can take 50 to 500 microliter volume in step of 10 is called
| a | 50 microliter fixed volume micropipette |
| b | 500 microliter fixed volume micropipette |
| c | step pipette |
| d | variable volume pipette |
3. Following is not required during pipetting serum with micropipette
| a | micropipette |
| b | tip |
| c | gloves |
| d | syringe |
3. Micropitte have following push-button stops
| a | 1 |
| b | 2 |
| c | 3 |
| d | 4 |
4. Removal of tips from micropipette require ideally
| a | 1 hand |
| b | 2 hands |
| c | none of the above |
| d | all of the above |
5. Plastic tips should be discarded in
| a | red bag |
| b | blue bag |
| c | black bag |
| d | yellow bag |